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Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals.
- Source :
-
Journal of neurophysiology [J Neurophysiol] 2014 Mar; Vol. 111 (5), pp. 1132-49. Date of Electronic Publication: 2013 Dec 18. - Publication Year :
- 2014
-
Abstract
- Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.
Details
- Language :
- English
- ISSN :
- 1522-1598
- Volume :
- 111
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of neurophysiology
- Publication Type :
- Academic Journal
- Accession number :
- 24353300
- Full Text :
- https://doi.org/10.1152/jn.00785.2013