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The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
- Source :
-
Development (Cambridge, England) [Development] 2014 Jan; Vol. 141 (1), pp. 73-82. - Publication Year :
- 2014
-
Abstract
- Specialized microenvironments, or niches, provide signaling cues that regulate stem cell behavior. In the Drosophila testis, the JAK-STAT signaling pathway regulates germline stem cell (GSC) attachment to the apical hub and somatic cyst stem cell (CySC) identity. Here, we demonstrate that chickadee, the Drosophila gene that encodes profilin, is required cell autonomously to maintain GSCs, possibly facilitating localization or maintenance of E-cadherin to the GSC-hub cell interface. Germline specific overexpression of Adenomatous Polyposis Coli 2 (APC2) rescued GSC loss in chic hypomorphs, suggesting an additive role of APC2 and F-actin in maintaining the adherens junctions that anchor GSCs to the niche. In addition, loss of chic function in the soma resulted in failure of somatic cyst cells to maintain germ cell enclosure and overproliferation of transit-amplifying spermatogonia.
- Subjects :
- Actins metabolism
Adherens Junctions metabolism
Animals
Cadherins metabolism
Cell Differentiation
Cell Lineage
Cell Proliferation
Drosophila Proteins biosynthesis
Drosophila Proteins genetics
Drosophila melanogaster cytology
Gene Expression Regulation, Developmental
Male
Profilins genetics
Protein Binding
RNA Interference
RNA, Small Interfering genetics
Signal Transduction genetics
Spermatogonia cytology
Spermatogonia metabolism
Spermatozoa cytology
Stem Cell Niche
Tumor Suppressor Proteins biosynthesis
Drosophila Proteins metabolism
Drosophila melanogaster metabolism
Profilins metabolism
Spermatozoa metabolism
Stem Cells metabolism
Tumor Suppressor Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 141
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 24346697
- Full Text :
- https://doi.org/10.1242/dev.101931