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Determination of matrix metalloproteinase 9 (MMP-9) protein expression in laryngeal squamous cell carcinomas based on digital image analysis.
- Source :
-
Journal of B.U.ON. : official journal of the Balkan Union of Oncology [J BUON] 2013 Oct-Dec; Vol. 18 (4), pp. 977-81. - Publication Year :
- 2013
-
Abstract
- Purpose: Matrix metalloproteinases (MMPs) is a superfamily of proteins involved in angiogenesis and metastatic tissue invasion in many cancers. Overexpression of MMP- 9 has been detected in significant proportions of laryngeal squamous cell carcinomas (LSCCs), but its prognostic impact remains unclear. In this study we performed a digital image analysis for analyzing MMP-9 protein expression in a series of LSCCs correlating them with clinicopathological factors.<br />Methods: MMP-9 protein expression level was determined immunohistochemically in 30 tissue sections surgically derived from patients (21 male and 9 female) with LSCC. Using digital image analysis, we measured their corresponding protein expression levels (staining intensity/S.I. range values 0-255).<br />Results: Moderate and high MMP-9 protein expression levels (grouping as 2+/3+ overexpression) were detected in 19/30 (63.3%) cases. Statistical significance was observed correlating stage with SI (p=0.02), whereas a borderline association with differentiation grade of the examined tumors was also registered (p=0.05). Interestingly, high levels of MMP-9 expression were observed in cases that demonstrated a significant level of inflammatory (predominantly lymphocytic) infiltration.<br />Conclusion: MMP-9 protein overactivation is a frequent and significant genetic event in LSCC, correlating with its biological behavior (increased TNM stage). MMP-9 seems to mediate an epithelial-stromal intra-reaction correlating also with induction of specific inflammation pathways.
- Subjects :
- Aged
Carcinoma, Squamous Cell pathology
Cell Differentiation
Chi-Square Distribution
Female
Head and Neck Neoplasms pathology
Humans
Image Interpretation, Computer-Assisted
Immunohistochemistry
Laryngeal Neoplasms pathology
Male
Middle Aged
Neoplasm Staging
Predictive Value of Tests
Prognosis
Squamous Cell Carcinoma of Head and Neck
Up-Regulation
Biomarkers, Tumor analysis
Carcinoma, Squamous Cell enzymology
Head and Neck Neoplasms enzymology
Laryngeal Neoplasms enzymology
Matrix Metalloproteinase 9 analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1107-0625
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of B.U.ON. : official journal of the Balkan Union of Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 24344026