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Absence of a role for interleukin-13 in inflammatory bowel disease.
- Source :
-
European journal of immunology [Eur J Immunol] 2014 Feb; Vol. 44 (2), pp. 370-85. - Publication Year :
- 2014
-
Abstract
- IL-13 has been implicated in the pathogenesis of ulcerative colitis (UC), and may have a role in animal models of gut fibrosis. We studied the involvement of IL-13 in inflammation and fibrosis in UC and Crohn's disease (CD). Intestinal biopsies and anti-CD3/CD28- or anti-CD2/CD28-stimulated lamina propria mononuclear cells from UC and CD patients and control subjects were cultured, and IL-13, IL-4, IL-5, IL-17A and IFN-γ production was measured. Mucosal IL-13-producing cells were characterised by flow cytometry. Gut explants from strictured CD, non-strictured CD and healthy donors were cultured ex vivo, and secreted IL-13, IL-1β and collagen were measured. IL-13 production by mucosal explants and activated lamina propria mononuclear cells did not differ between CD, UC and control subjects, and was at least a log lower than IFN-γ and IL-17A. IL-13-producing cells, and in particular natural killer T cells, were uniformly low in all groups. IL-4 and IL-5 were undetectable in culture supernatants. Explants of CD strictures produced low amounts of IL-13, whereas IL-1β and collagen were elevated. We could not confirm that UC or strictured CD are associated with elevated IL-13 production. These data suggest that an anti-IL-13 Ab would not be an appropriate therapeutic strategy in inflammatory bowel disease.<br /> (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Adolescent
Adult
Aged
CD28 Antigens immunology
CD3 Complex immunology
Colitis, Ulcerative pathology
Crohn Disease pathology
Fibrosis pathology
Humans
Inflammation pathology
Inflammatory Bowel Diseases pathology
Interferon-gamma immunology
Interleukin-13 Receptor alpha1 Subunit immunology
Interleukin-13 Receptor alpha2 Subunit immunology
Intestinal Mucosa immunology
Intestinal Mucosa pathology
Intestines immunology
Intestines pathology
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear pathology
Macrophages immunology
Macrophages pathology
Middle Aged
Mucous Membrane immunology
Mucous Membrane pathology
Natural Killer T-Cells immunology
Th2 Cells immunology
Transforming Growth Factor beta1 immunology
Young Adult
Colitis, Ulcerative immunology
Crohn Disease immunology
Fibrosis immunology
Inflammation immunology
Inflammatory Bowel Diseases immunology
Interleukin-13 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 44
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 24338958
- Full Text :
- https://doi.org/10.1002/eji.201343524