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Sensitive and rapid detection of Chlamydia trachomatis by recombinase polymerase amplification directly from urine samples.
- Source :
-
The Journal of molecular diagnostics : JMD [J Mol Diagn] 2014 Jan; Vol. 16 (1), pp. 127-35. - Publication Year :
- 2014
-
Abstract
- Chlamydia trachomatis is the most common sexually transmitted human pathogen. Infection results in minimal to no symptoms in approximately two-thirds of women and therefore often goes undiagnosed. C. trachomatis infections are a major public health concern because of the potential severe long-term consequences, including an increased risk of ectopic pregnancy, chronic pelvic pain, and infertility. To date, several point-of-care tests have been developed for C. trachomatis diagnostics. Although many of them are fast and specific, they lack the required sensitivity for large-scale application. We describe a rapid and sensitive form of detection directly from urine samples. The assay uses recombinase polymerase amplification and has a minimum detection limit of 5 to 12 pathogens per test. Furthermore, it enables detection within 20 minutes directly from urine samples without DNA purification before the amplification reaction. Initial analysis of the assay from clinical patient samples had a specificity of 100% (95% CI, 92%-100%) and a sensitivity of 83% (95% CI, 51%-97%). The whole procedure is fairly simple and does not require specific machinery, making it potentially applicable in point-of-care settings.<br /> (Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Chlamydia Infections genetics
Chlamydia trachomatis enzymology
DNA, Bacterial analysis
DNA-Directed DNA Polymerase chemistry
Diacylglycerol Cholinephosphotransferase genetics
Female
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) genetics
Humans
Molecular Diagnostic Techniques methods
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Recombinases chemistry
Sensitivity and Specificity
Sexually Transmitted Diseases, Bacterial genetics
Chlamydia Infections diagnosis
Chlamydia Infections urine
Chlamydia trachomatis genetics
Sexually Transmitted Diseases, Bacterial diagnosis
Sexually Transmitted Diseases, Bacterial urine
Subjects
Details
- Language :
- English
- ISSN :
- 1943-7811
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of molecular diagnostics : JMD
- Publication Type :
- Academic Journal
- Accession number :
- 24331366
- Full Text :
- https://doi.org/10.1016/j.jmoldx.2013.08.003