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Substance P-containing terminals in synaptic contact with cholinergic neurons in the neostriatum and basal forebrain: a double immunocytochemical study in the rat.
- Source :
-
Brain research [Brain Res] 1986 Nov 12; Vol. 397 (2), pp. 279-89. - Publication Year :
- 1986
-
Abstract
- Antibodies against substance P and choline acetyltransferase (ChAT) have been used in a sequential double-immunocytochemical ultrastructural study of the rat forebrain. The peroxidase-anti-peroxidase procedure was used for both antigens, however, two different substrates for the peroxidase reactions were used. The substance P-immunoreactive sites were first localized using 3,3'-diaminobenzidine as the substrate, then the ChAT-immunoreactive sites were localized using benzidine dihydrochloride. The reaction product formed by the two substrates was distinguishable in both the light and electron microscopes. Using this procedure, the cell bodies and proximal dendrites of identified cholinergic neurons in the neostriatum were found to receive symmetrical synaptic input from substance P-immunoreactive boutons. A similar pattern of substance P-immunoreactive synaptic input was observed onto magnocellular basal forebrain cholinergic neurons in the ventral pallidum and ventromedial globus pallidus. In both the striatum and basal forebrain substance P-immunoreactive boutons were also seen in contact with structures that did not display ChAT immunoreactivity.
- Subjects :
- Animals
Benzidines
Choline O-Acetyltransferase metabolism
Cholinergic Fibers enzymology
Cholinergic Fibers ultrastructure
Corpus Striatum ultrastructure
Female
Frontal Lobe ultrastructure
Histocytochemistry
Horseradish Peroxidase
Immunoenzyme Techniques
Microscopy, Electron
Rats
Synapses ultrastructure
Cholinergic Fibers metabolism
Corpus Striatum metabolism
Frontal Lobe metabolism
Substance P metabolism
Synapses metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 397
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 2432992
- Full Text :
- https://doi.org/10.1016/0006-8993(86)90629-3