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Localization of actin in mammalian spermatozoa: a comparison of eight species.

Authors :
Flaherty SP
Winfrey VP
Olson GE
Source :
The Anatomical record [Anat Rec] 1986 Dec; Vol. 216 (4), pp. 504-15.
Publication Year :
1986

Abstract

The distribution of monomeric and polymeric actin in spermatozoa from the bull, boar, rabbit, human, rat, mouse, golden hamster, and guinea pig has been examined by using a monoclonal antiactin antibody and NBD-phallacidin. Actin was present in sperm from each species. When the monoclonal antibody was used, there was a species-specific distribution and intensity of fluorescence, but no generalized pattern. Specific fluorescence was noted in the neck and principal piece of human sperm; in the postacrosomal region, neck, and midpiece of bull and boar sperm; in the postacrosomal region, neck, and principal/equatorial segment border of rabbit sperm; in the neck region of hamster sperm; and in the neck, midpiece, and principal piece of rat, mouse, and guinea pig sperm. Sperm from all eight species displayed no specific fluorescence with NBD-phallacidin, indicating that actin was present in a nonfilamentous form. SDS extracts of sperm were analyzed by SDS-PAGE and Western blotting; in sperm from each species, a 42-kD protein with specific affinity for the monoclonal antibody was present.

Details

Language :
English
ISSN :
0003-276X
Volume :
216
Issue :
4
Database :
MEDLINE
Journal :
The Anatomical record
Publication Type :
Academic Journal
Accession number :
2432804
Full Text :
https://doi.org/10.1002/ar.1092160407