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Bacterial infection (BI)-INDEX: an improved and simplified rapid flow cytometric bacterial infection marker.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2014 Feb; Vol. 78 (2), pp. 116-26. Date of Electronic Publication: 2013 Oct 31. - Publication Year :
- 2014
-
Abstract
- The aim of this study was to develop a rapid and simple flow cytometric bacterial infection marker. In this prospective comparative study, quantitative flow cytometric analysis of CD10, CD35, CD66b, CD282, and MHC Class I molecules on human neutrophils, monocytes, and B-lymphocytes from 141 hospitalized febrile patients with suspected infection and from 50 healthy controls was performed. We developed a flow cytometric marker of local and systemic bacterial infections, designated "bacterial infection (BI)-INDEX", incorporating the quantitative analysis of CD10, CD35, MHCI, CD66b, and CD282 on neutrophils, monocytes, and B-lymphocytes, which displayed 90% sensitivity and 96% specificity in distinguishing between microbiologically confirmed bacterial (n = 31) and viral infections (n = 27) within a 1-h time-frame. We propose that our novel rapid BI-INDEX test will be useful in assisting physicians to ascertain whether antibiotic treatment is required, thus limiting unnecessary antimicrobial usage.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Antigens, Surface metabolism
B-Lymphocytes metabolism
Bacterial Infections microbiology
Biomarkers metabolism
Case-Control Studies
Diagnosis, Differential
Female
Humans
Male
Middle Aged
Monocytes metabolism
Neutrophils metabolism
ROC Curve
Receptors, Cell Surface metabolism
Reproducibility of Results
Young Adult
Bacterial Infections diagnosis
Bacterial Infections metabolism
Flow Cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 78
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 24315569
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2013.10.016