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Bacterial infection (BI)-INDEX: an improved and simplified rapid flow cytometric bacterial infection marker.

Authors :
Nuutila J
Jalava-Karvinen P
Hohenthal U
Kotilainen P
Pelliniemi TT
Nikoskelainen J
Lilius EM
Source :
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2014 Feb; Vol. 78 (2), pp. 116-26. Date of Electronic Publication: 2013 Oct 31.
Publication Year :
2014

Abstract

The aim of this study was to develop a rapid and simple flow cytometric bacterial infection marker. In this prospective comparative study, quantitative flow cytometric analysis of CD10, CD35, CD66b, CD282, and MHC Class I molecules on human neutrophils, monocytes, and B-lymphocytes from 141 hospitalized febrile patients with suspected infection and from 50 healthy controls was performed. We developed a flow cytometric marker of local and systemic bacterial infections, designated "bacterial infection (BI)-INDEX", incorporating the quantitative analysis of CD10, CD35, MHCI, CD66b, and CD282 on neutrophils, monocytes, and B-lymphocytes, which displayed 90% sensitivity and 96% specificity in distinguishing between microbiologically confirmed bacterial (n = 31) and viral infections (n = 27) within a 1-h time-frame. We propose that our novel rapid BI-INDEX test will be useful in assisting physicians to ascertain whether antibiotic treatment is required, thus limiting unnecessary antimicrobial usage.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1879-0070
Volume :
78
Issue :
2
Database :
MEDLINE
Journal :
Diagnostic microbiology and infectious disease
Publication Type :
Academic Journal
Accession number :
24315569
Full Text :
https://doi.org/10.1016/j.diagmicrobio.2013.10.016