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miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes.
- Source :
-
Virology [Virology] 2014 Jan 05; Vol. 448, pp. 210-6. Date of Electronic Publication: 2013 Oct 29. - Publication Year :
- 2014
-
Abstract
- A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes.<br /> (© 2013 Published by Elsevier Inc.)
- Subjects :
- Cell Proliferation
Foreskin cytology
Foreskin metabolism
Foreskin virology
Human papillomavirus 16 genetics
Humans
Keratinocytes cytology
Keratinocytes virology
Male
MicroRNAs metabolism
Oncogene Proteins, Viral genetics
Papillomavirus E7 Proteins genetics
Papillomavirus Infections metabolism
Papillomavirus Infections physiopathology
Papillomavirus Infections virology
Repressor Proteins genetics
Human papillomavirus 16 metabolism
Keratinocytes metabolism
MicroRNAs genetics
Oncogene Proteins, Viral metabolism
Papillomavirus E7 Proteins metabolism
Papillomavirus Infections genetics
Repressor Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 448
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 24314651
- Full Text :
- https://doi.org/10.1016/j.virol.2013.10.014