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PCR as a screening test for invasive aspergillosis in haematological patients: a pilot study.
- Source :
-
Mycopathologia [Mycopathologia] 2014 Feb; Vol. 177 (1-2), pp. 111-4. Date of Electronic Publication: 2013 Dec 06. - Publication Year :
- 2014
-
Abstract
- Invasive aspergillosis is a leading cause of morbidity and mortality in immunocompromised patients, particularly in individuals with haematological malignancy and in haematopoietic stem cell transplant recipients. Nowadays, the galactomannan (GM) assay has been widely used as an indication of invasive aspergillosis, even though the test is known to generate false-positive results. The aim of this study was to compare the performance of GM and real-time PCR (qPCR) to detected Aspergillus in blood samples obtained from high-risk haematological patients. Haematological patients were screened twice weekly with GM testing, which was performed by the Platelia ELISA kit. An additional sample of whole blood (4 ml) was obtained for the purpose of qPCR testing. Sixty-four samples from 12 patients with haematopoietic stem cell transplant or haematological malignancy were studied. The overall accordance between GM and qPCR tests was 96.9 % (62 samples). Only two samples showed contradictory results, with positive GM test and negative real-time PCR results. Based on the high concordance between GM and qPCR in terms of negative results, the main utility of qPCR could be in the confirmation of positive results seen with GM testing.
- Subjects :
- Adult
Aspergillosis blood
Aspergillus isolation & purification
DNA, Fungal analysis
False Positive Reactions
Female
Galactose analogs & derivatives
Hematologic Neoplasms microbiology
Hematopoietic Stem Cell Transplantation adverse effects
Humans
Immunocompromised Host
Male
Mannans analysis
Mass Screening
Middle Aged
Pilot Projects
Real-Time Polymerase Chain Reaction
Young Adult
Aspergillosis diagnosis
Aspergillus genetics
DNA, Fungal genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0832
- Volume :
- 177
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Mycopathologia
- Publication Type :
- Academic Journal
- Accession number :
- 24309908
- Full Text :
- https://doi.org/10.1007/s11046-013-9718-0