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Diversity of epothilone producers among Sorangium strains in producer-positive soil habitats.
- Source :
-
Microbial biotechnology [Microb Biotechnol] 2014 Mar; Vol. 7 (2), pp. 130-41. Date of Electronic Publication: 2013 Dec 06. - Publication Year :
- 2014
-
Abstract
- Large-scale surveys show that the anti-tumour compounds known as epothilones are produced by only a small proportion of Sorangium strains, thereby greatly hampering the research and development of these valuable compounds. In this study, to investigate the niche diversity of epothilone-producing Sorangium strains, we re-surveyed four soil samples where epothilone producers were previously found. Compared with the < 2.5% positive strains collected from different places, epothilone producers comprised 25.0-75.0% of the Sorangium isolates in these four positive soil samples. These sympatric epothilone producers differed not only in their 16S rRNA gene sequences and morphologies but also in their production of epothilones and biosynthesis genes. A further exploration of 14 soil samples collected from a larger area around a positive site showed a similar high positive ratio of epothilone producers among the Sorangium isolates. The present results suggest that, in an area containing epothilone producers, the long-term genetic variations and refinements resulting from selective pressure form a large reservoir of epothilone-producing Sorangium strains with diverse genetic compositions.<br /> (© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)
- Subjects :
- Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Ecosystem
Metabolic Networks and Pathways genetics
Molecular Sequence Data
Myxococcales classification
Myxococcales genetics
Myxococcales isolation & purification
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Antineoplastic Agents metabolism
Epothilones metabolism
Myxococcales metabolism
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1751-7915
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbial biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 24308800
- Full Text :
- https://doi.org/10.1111/1751-7915.12103