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[Detection of cationic proteins and peroxidase in semithin sections].

Authors :
PigarevskiÄ­ VE
Mazing IuA
Seliverstova VG
Source :
Arkhiv patologii [Arkh Patol] 1986; Vol. 48 (8), pp. 77-80.
Publication Year :
1986

Abstract

A method of histochemical identification of cationic proteins, peroxidase, viable and killed bacteria in semithin sections of biopsy samples is suggested. The method is based on dying of araldite-embedded semithin sections with a buffered alcohol solution of fast green (pH 8.15) and on peroxidase identification with O-dianisidine. The method suggested can be used for distinguishing occult forms of infectious gastritis, evaluation of secretory and barrier function of gastric epithelium, selective dying of leucocytes in tissues, revealing of viable and non-viable bacteria in the infective foci.

Details

Language :
Russian
ISSN :
0004-1955
Volume :
48
Issue :
8
Database :
MEDLINE
Journal :
Arkhiv patologii
Publication Type :
Academic Journal
Accession number :
2430554