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Immunization using GroEL decreases Clostridium difficile intestinal colonization.
- Source :
-
PloS one [PLoS One] 2013 Nov 26; Vol. 8 (11), pp. e81112. Date of Electronic Publication: 2013 Nov 26 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Clostridium difficile is a pathogen which is responsible for diarrhea and colitis, particularly after treatment with antibiotics. Clinical signs are mainly due to two toxins, TcdA and TcdB. However, the first step of pathogenesis is the colonization process. We evaluated C. difficile surface proteins as vaccine antigens in the hamster model to prevent intestinal colonization. This vaccination induced a partial protection of hamsters against death after a C. difficile challenge. A proteomic analysis of animal sera allowed us to identify proteins which could be responsible for the protection observed. Among these proteins, we identified the GroEL heat shock protein. To confirm the role of the specific GroEL antibodies in the delayed C. difficile colonization of hamsters, we performed an immunization assay in a mouse model. After intranasal immunization with the recombinant protein GroEL, we observed a lower C. difficile intestinal colonization in the immunized group as compared to the control group.
- Subjects :
- Animals
Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Antibody Specificity immunology
Bacterial Proteins metabolism
Bacterial Vaccines immunology
Cell Wall immunology
Cell Wall metabolism
Chaperonin 60 metabolism
Clostridioides difficile metabolism
Cricetinae
Disease Models, Animal
Enterocolitis, Pseudomembranous immunology
Enterocolitis, Pseudomembranous microbiology
Enterocolitis, Pseudomembranous mortality
Female
Immunization
Mice
Proteomics methods
Recombinant Proteins immunology
Bacterial Proteins immunology
Chaperonin 60 immunology
Clostridioides difficile immunology
Intestines immunology
Intestines microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24303034
- Full Text :
- https://doi.org/10.1371/journal.pone.0081112