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Bromodeoxyuridine (BrdU)-label-retaining cells in mouse terminal bronchioles.
- Source :
-
Histology and histopathology [Histol Histopathol] 2014 May; Vol. 29 (5), pp. 659-68. Date of Electronic Publication: 2013 Dec 04. - Publication Year :
- 2014
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Abstract
- Adult male mice were continuously treated with bromodeoxyuridine (BrdU) for 1, 2, or 4 weeks by an osmotic pump. To detect BrdU-label-retaining cells (LRCs), putative progenitor/stem cells, other animals were continuously treated with BrdU for 2 weeks, and were then kept without any treatments for 2, 6, or 18 months. The lungs were fixed with 4% paraformaldehyde, and were paraffin-embedded. We observed terminal bronchioles with BrdU immunostaining alone or with BrdU immunostaining accompanying immunostaining for Clara cell secretory protein (CCSP), forkhead box protein J1 (FoxJ1), or calcitonin gene-related peptide (CGRP). The average incidences of BrdU-incorporated cells in the terminal bronchioles after 1, 2, and 4 weeks of continuous BrdU infusion were 6.2%, 11.9%, and 23.1%, respectively. Most BrdU-incorporated cells in these periods were CCSP-immunoreactive (91.7%, 91.3%, and 88.2%, respectively), which means progenitor function of Clara cells. FoxJ1-immunoreactive BrdU-incorporated cells were fewer (5.4%, 3.0%, 2.7%, respectively). The average incidences of BrdU-LRCs in the terminal bronchioles after 2, 6, and 18 months were 7.2%, 4.3, and 2.7%, respectively. Most BrdU-LRCs were CCSP-immunoreactive (91.0%, 92.7%, and 89.6%, respectively), and FoxJ1-immunoreactive BrdU-LRCs were fewer (6.0%, 5.7%, and 2.1%, respectively). CGRP-positive BrdU-incorporated cells were occasional. CGRP-positive BrdU-LRCs were detected in 17.6% of neuroepithelial bodies (NEBs) at 2 months, but disappeared at 6 months. BrdU-positive stem cell candidates, which locate at the brochiolo-alveolar duct junction or cover NEB, were few throughout this study. In conclusion, in the lungs treated only with BrdU, CCSP-immunoreactive cells are important to maintain homeostasis in the terminal bronchiolar epithelium.
- Subjects :
- Animals
Bromodeoxyuridine administration & dosage
Calcitonin Gene-Related Peptide metabolism
Forkhead Transcription Factors metabolism
Immunohistochemistry
Male
Mice
Mice, Inbred ICR
Neuroepithelial Bodies cytology
Neuroepithelial Bodies metabolism
Pulmonary Alveoli cytology
Pulmonary Alveoli metabolism
Respiratory Mucosa cytology
Respiratory Mucosa metabolism
Time Factors
Uteroglobin metabolism
Adult Stem Cells cytology
Adult Stem Cells metabolism
Bromodeoxyuridine pharmacokinetics
Bronchioles cytology
Bronchioles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1699-5848
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Histology and histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 24301684
- Full Text :
- https://doi.org/10.14670/HH-29.10.659