Expression of Arabidopsis thaliana xylose isomerase gene and its effect on ethanol production in Flammulina velutipes.

Authors :: Maehara T
Takabatake K
Kaneko S
Source :: Fungal biology [Fungal Biol] 2013 Nov-Dec; Vol. 117 (11-12), pp. 776-82. Date of Electronic Publication: 2013 Oct 12.
Publication Year :: 2013
Abstract: To improve the pentose fermentation rate in Flammulina velutipes, the putative xylose isomerase (XI) gene from Arabidopsis thaliana was cloned and introduced into F. velutipes and the gene expression was evaluated in transformants. mRNA expression of the putative XI gene and XI activity were observed in two transformants, indicating that the putative gene from A. thaliana was successfully expressed in F. velutipes as a xylose isomerase. In addition, ethanol production from xylose was increased in the recombinant strains. This is the first report demonstrating the possibility of using plant genes as candidates for improving the characteristics of F. velutipes.<br /> (Copyright © 2013 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.)

Subjects :: Aldose-Ketose Isomerases genetics
Arabidopsis genetics
Fermentation
Flammulina genetics
Gene Expression Profiling
RNA, Fungal analysis
RNA, Fungal genetics
RNA, Messenger analysis
RNA, Messenger genetics
Recombinant Proteins biosynthesis
Recombinant Proteins genetics
Xylose metabolism
Aldose-Ketose Isomerases biosynthesis
Arabidopsis enzymology
Ethanol metabolism
Flammulina enzymology
Flammulina metabolism

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