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Evaluation of diagnostic potential of Rv3803c and Rv2626c recombinant antigens in TB endemic country Pakistan.
- Source :
-
Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2014; Vol. 35 (2), pp. 120-9. - Publication Year :
- 2014
-
Abstract
- To overcome and eliminate tuberculosis (TB), definitive, reliable, and rapid diagnosis is mandatory. Presently, the diagnostic potential of acute and latent stage TB specific antigens i.e., Rv3803c and Rv2626c was determined. Immunogenic recombinant genes of Rv3803c and Rv2626c antigens were cloned in bacterial expression vector pET23b and expressed product was purified. The homogeneity and structural integrity was confirmed by Western blot analysis. Diagnostic potential of Rv3803c and Rv2626c antigens was analyzed using the sera of 140 active TB patients (AFB smear positive) by indirect ELISA. Ten patients of leprosy and 94 healthy individuals were taken as disease and normal control respectively. The data was analyzed using R statistical package. The sensitivity and specificity of Rv3803c in active TB patients was of 69.3% and 76.4% respectively with an area under ROC curve of 0.77, whereas sensitivity and specificity of Rv2626c 77.1% and 85.1%, respectively. The area under ROC curve of Rv2626c was 0.89 which was significantly higher than Rv3803c (p < 0.0001). Recombinant antigens Rv3803c and Rv2626c have potential to be used as diagnostic markers for TB and need to evaluate with other antigens for differential diagnosis of TB.
- Subjects :
- Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Antigens, Bacterial blood
Antigens, Bacterial genetics
Bacterial Proteins genetics
Bacterial Proteins immunology
Enzyme-Linked Immunosorbent Assay
Galactosyltransferases genetics
Galactosyltransferases immunology
Humans
Mycobacterium tuberculosis genetics
Mycobacterium tuberculosis immunology
Pakistan
Recombinant Proteins analysis
Recombinant Proteins genetics
Recombinant Proteins immunology
Sensitivity and Specificity
Tuberculosis blood
Tuberculosis immunology
Antigens, Bacterial immunology
Bacterial Proteins analysis
Galactosyltransferases analysis
Tuberculosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4230
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunoassay & immunochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24295176
- Full Text :
- https://doi.org/10.1080/15321819.2013.824897