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Mice immunogenicity after vaccination by DNA vaccines containing individual genes of a new type of reovirus.
- Source :
-
Acta virologica [Acta Virol] 2013; Vol. 57 (4), pp. 397-404. - Publication Year :
- 2013
-
Abstract
- In this study, we investigated humoral and cellular immune responses in mice to DNA vaccines containing individual S or M genes of a new type of reovirus (nRV) isolate from a severe acute respiratory syndrome (SARS) patient in Beijing, China. Mice were immunized intramuscularly (i.m.) with 100 μg of S1, S2, S3, S4, M1, M2, and M3 DNA vaccine each 4 times in 2-week intervals and assayed for humoral IgG, IgG1, IgG2, and IgG2b antibodies by ELISA and for cellular immune response, particularly IFN-γ induction by ELISpot assay. Moreover, CD4+ and CD8+ T cell levels in peripheral blood mononuclear cells were assayed by flow cytometry. We found that all DNA vaccines induced IgG antibodies, predominantly of the IgG2a class and S3 DNA vaccine was the strongest inducer. M2 and S3 DNA vaccines elicited Th1- and Th2-based immune responses, respectively, while S1 and M3 DNA vaccines induced a mixed Th1/Th2 response. M1, S2, and S4 DNA vaccines were poorly immunogenic. To our knowledge, this is the first report characterizing mammalian reovirus DNA vaccines applied to a mouse model.
- Subjects :
- Animals
Antibodies, Viral immunology
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Female
Humans
Mice
Mice, Inbred BALB C
Orthoreovirus, Mammalian genetics
Orthoreovirus, Mammalian isolation & purification
Reoviridae Infections virology
Vaccination
Vaccines, DNA administration & dosage
Vaccines, DNA genetics
Viral Proteins genetics
Viral Vaccines administration & dosage
Viral Vaccines genetics
Orthoreovirus, Mammalian immunology
Reoviridae Infections immunology
Vaccines, DNA immunology
Viral Proteins immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0001-723X
- Volume :
- 57
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta virologica
- Publication Type :
- Academic Journal
- Accession number :
- 24294952
- Full Text :
- https://doi.org/10.4149/av_2013_04_397