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Analysis of bronchoalveolar lavage fluid from bleomycin-induced pulmonary fibrosis in hamsters.
- Source :
-
Toxicologic pathology [Toxicol Pathol] 1986; Vol. 14 (2), pp. 149-57. - Publication Year :
- 1986
-
Abstract
- The purpose of this study was to analyze the cellular and noncellular components of bronchoalveolar lavage fluid (BALF) at varying times during the development of pulmonary fibrosis induced by bleomycin. Hamsters were killed and lavaged in situ following the administration of a single intratracheal injection of 1 unit of bleomycin or an equivalent volume of sterile isotonic saline. The results show that the total cell counts in the BALF of bleomycin-treated hamsters, as compared with controls, were increased 7.7, 4.4, 2.4, 1.6, and 1.9-fold at 2, 4, 7, 14, and 21 days after treatment, respectively. The predominant cell types in the BALF of control animals were macrophages which constituted 84% of the total cells, followed by lymphocytes, 11%. The predominant cell types in the BALF of bleomycin-treated animals were polymorphonuclear leukocytes (PMN) which constituted 65% at two days and approximately 50% of the total at 4, 7, and 14 days; at 21 days macrophages were the predominant cell type constituting 50%, followed by lymphocytes at 30%. However, the total number of lymphocytes was not increased at 21 days compared to previous times. The noncellular protein content of BALF from bleomycin-treated hamsters, an index of pulmonary vascular permeability, was increased to 224, 559, 637, and 270% of control (2.7 mg/lung) at 2, 4, 7, and 14 days after treatment, respectively, and returned to control levels at 21 days. The acid phosphatase activity in the supernatant of BALF of bleomycin-treated animals was significantly increased to 181, 181, 199, 176, and 125% of control (258 units/lung) at 2, 4, 7, 14, and 21 days, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Acid Phosphatase metabolism
Animals
Cricetinae
Glucuronidase metabolism
Histamine metabolism
Leukocyte Count drug effects
Lymphocytes drug effects
Macrophages drug effects
Male
Mesocricetus
Neutrophils drug effects
Prostaglandins E metabolism
Proteins metabolism
Pulmonary Alveoli pathology
Pulmonary Fibrosis chemically induced
Pulmonary Fibrosis pathology
Therapeutic Irrigation
Bleomycin toxicity
Pulmonary Alveoli metabolism
Pulmonary Fibrosis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0192-6233
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Toxicologic pathology
- Publication Type :
- Academic Journal
- Accession number :
- 2429361
- Full Text :
- https://doi.org/10.1177/019262338601400202