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A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus.
- Source :
-
Archives of virology [Arch Virol] 2014 May; Vol. 159 (5), pp. 1067-77. Date of Electronic Publication: 2013 Dec 01. - Publication Year :
- 2014
-
Abstract
- Infections with influenza and respiratory syncytial virus (RSV) rank high among the most common human respiratory diseases worldwide. Previously, we developed a replication-incompetent influenza virus by replacing the coding sequence of the PB2 gene, which encodes one of the viral RNA polymerase subunits, with that of a reporter gene. Here, we generated a PB2-knockout recombinant influenza virus expressing the F protein of RSV (PB2-RSVF virus) and tested its potential as a bivalent vaccine. In mice intranasally immunized with the PB2-RSVF virus, we detected high levels of antibodies against influenza virus, but not RSV. PB2-RSVF virus-immunized mice were protected from a lethal challenge with influenza virus but experienced severe body weight loss when challenged with RSV, indicating that PB2-RSVF vaccination enhanced RSV-associated disease. These results highlight one of the difficulties of developing an effective bivalent vaccine against influenza virus and RSV infections.
- Subjects :
- Animals
Female
Gene Expression Regulation, Viral
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Respiratory Syncytial Virus Infections prevention & control
Respiratory Syncytial Viruses metabolism
Vaccines, Synthetic immunology
Virus Replication
Influenza Vaccines immunology
Orthomyxoviridae Infections prevention & control
Respiratory Syncytial Viruses immunology
Viral Fusion Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 159
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 24292020
- Full Text :
- https://doi.org/10.1007/s00705-013-1932-z