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Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.
- Source :
-
BMB reports [BMB Rep] 2014 May; Vol. 47 (5), pp. 292-7. - Publication Year :
- 2014
-
Abstract
- Age-related macular degeneration (AMD) is the leading cause of blindness in the world. Evidence indicates that the suppression of the ubiquitin-proteasome system (UPS) contributes to the accumulation of toxic proteins and inflammation in retinal pigment epithelium (RPE), the functional abnormalities and/or the degeneration of which are believed to be the initiators and major pathologies of AMD. To identify new protein associations with the altered UPS in AMD, we used LC-ESI-MS/MS to perform a proteomic analysis of the aqueous humor (AH) of AMD patients and matched control subjects. Six UPS-related proteins were present in the AH of the patients and control subjects. Four of the proteins, including 26S proteasome non-ATPase regulatory subunit 1 (Rpn2), were increased in patients, according to semi-quantitative proteomic profiling. An LC-MRM assay revealed a significant increase of Rpn2 in 15 AMD patients compared to the control subjects, suggesting that this protein could be a biomarker for AMD.
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 47
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 24286321
- Full Text :
- https://doi.org/10.5483/bmbrep.2014.47.5.193