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Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice.
- Source :
-
The FEBS journal [FEBS J] 2014 Feb; Vol. 281 (3), pp. 894-915. Date of Electronic Publication: 2014 Jan 07. - Publication Year :
- 2014
-
Abstract
- Ca²⁺ homeostasis is required to maintain a delicate balance of cytosolic Ca²⁺ during normal and adverse growth conditions. Various Ca²⁺ transporters actively participate to maintain this delicate balance especially during abiotic stresses and developmental events in plants. In this study, we present a genome-wide account, detailing expression profiles, subcellular localization and functional analysis of rice Ca²⁺ transport elements. Exhaustive in silico data mining and analysis resulted in the identification of 81 Ca²⁺ transport element genes, which belong to various groups such as Ca²⁺-ATPases (pumps), exchangers, channels, glutamate receptor homologs and annexins. Phylogenetic analysis revealed that different Ca²⁺ transporters are evolutionarily conserved across different plant species. Comprehensive expression analysis by gene chip microarray and quantitative RT-PCR revealed that a substantial proportion of Ca²⁺ transporter genes were expressed differentially under abiotic stresses (salt, cold and drought) and reproductive developmental stages (panicle and seed) in rice. These findings suggest a possible role of rice Ca²⁺ transporters in abiotic stress and development triggered signaling pathways. Subcellular localization of Ca²⁺ transporters from different groups in Nicotiana benthamiana revealed their variable localization to different compartments, which could be their possible sites of action. Complementation of Ca²⁺ transport activity of K616 yeast mutant by Ca²⁺-ATPase OsACA7 and involvement in salt tolerance verified its functional behavior. This study will encourage detailed characterization of potential candidate Ca²⁺ transporters for their functional role in planta.<br /> (© 2013 FEBS.)
- Subjects :
- Biological Transport
Calcium-Transporting ATPases chemistry
Calcium-Transporting ATPases genetics
Calcium-Transporting ATPases metabolism
Cold Temperature
Droughts
Gene Duplication
Gene Expression Profiling
Genome-Wide Association Study
Green Fluorescent Proteins chemistry
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Inflorescence growth & development
Inflorescence metabolism
Oryza growth & development
Phylogeny
Plant Proteins chemistry
Plant Proteins genetics
Plants, Genetically Modified cytology
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins metabolism
Salinity
Seeds growth & development
Seeds metabolism
Nicotiana cytology
Nicotiana genetics
Nicotiana metabolism
Allostasis genetics
Calcium metabolism
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Oryza physiology
Plant Proteins metabolism
Stress, Physiological
Subjects
Details
- Language :
- English
- ISSN :
- 1742-4658
- Volume :
- 281
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The FEBS journal
- Publication Type :
- Academic Journal
- Accession number :
- 24286292
- Full Text :
- https://doi.org/10.1111/febs.12656