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Viral-restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens.
- Source :
-
Cellular immunology [Cell Immunol] 1986 Apr 15; Vol. 99 (1), pp. 279-86. - Publication Year :
- 1986
-
Abstract
- Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2Kb expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.
- Subjects :
- Animals
Cell Line
Cytotoxicity, Immunologic
Epitopes immunology
H-2 Antigens genetics
HLA Antigens genetics
HLA-A2 Antigen
Humans
L Cells immunology
Mice
Parainfluenza Virus 1, Human immunology
Recombinant Fusion Proteins genetics
Rhabdomyosarcoma
Vesicular stomatitis Indiana virus immunology
Antigens, Viral immunology
H-2 Antigens immunology
HLA Antigens immunology
Recombinant Fusion Proteins immunology
Recombinant Proteins immunology
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 99
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2428530
- Full Text :
- https://doi.org/10.1016/0008-8749(86)90235-2