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Biliary casts after liver transplantation: morphology and biochemical analysis.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2013 Nov 21; Vol. 19 (43), pp. 7772-7. - Publication Year :
- 2013
-
Abstract
- Aim: To investigate the pathogenesis of biliary casts after liver transplantation relative to their morphology and biochemical markers.<br />Methods: The microstructure of biliary casts was assessed using scanning electron microscopy and Hematoxylin and eosin staining assessed their histology. The expression levels of CD3, CD5, CD34, CD68 and CD79a in these biliary casts were evaluated immunohistochemically.<br />Results: Biliary casts differed widely in their microstructure, with some containing blood vessels positive for CD34 and collagen fibers with positive Masson staining. Large numbers of neutrophils and other inflammatory cells were present, but only on the edge of the biliary casts; although the boundaries were clear without crossover. None of the biliary casts contained T-lymphocytes, B-lymphocytes, macrophages and other inflammatory cells.<br />Conclusion: The microcostructure of biliary casts differed. Bacteria and acute rejection are not clearly related to their formation.
- Subjects :
- Adult
Aged
Antigens, CD analysis
Antigens, CD34 analysis
Antigens, Differentiation, Myelomonocytic analysis
Biliary Tract Diseases immunology
Biliary Tract Diseases pathology
Biomarkers analysis
Blood Vessels immunology
Blood Vessels ultrastructure
CD3 Complex analysis
CD5 Antigens analysis
CD79 Antigens analysis
Collagen analysis
Female
Humans
Immunohistochemistry
Male
Microscopy, Electron, Scanning
Middle Aged
Neutrophils immunology
Neutrophils ultrastructure
Bile Ducts immunology
Bile Ducts ultrastructure
Biliary Tract Diseases etiology
Liver Transplantation adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 19
- Issue :
- 43
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 24282366
- Full Text :
- https://doi.org/10.3748/wjg.v19.i43.7772