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Potassium nutrition of ectomycorrhizal Pinus pinaster: overexpression of the Hebeloma cylindrosporum HcTrk1 transporter affects the translocation of both K(+) and phosphorus in the host plant.
- Source :
-
The New phytologist [New Phytol] 2014 Feb; Vol. 201 (3), pp. 951-960. Date of Electronic Publication: 2013 Nov 27. - Publication Year :
- 2014
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Abstract
- Mycorrhizal associations are known to improve the hydro-mineral nutrition of their host plants. However, the importance of mycorrhizal symbiosis for plant potassium nutrition has so far been poorly studied. We therefore investigated the impact of the ectomycorrhizal fungus Hebeloma cylindrosporum on the potassium nutrition of Pinus pinaster and examined the involvement of the fungal potassium transporter HcTrk1. HcTrk1 transcripts and proteins were localized in ectomycorrhizas using in situ hybridization and EGFP translational fusion constructs. Importantly, an overexpression strategy was performed on a H. cylindrosporum endogenous gene in order to dissect the role of this transporter. The potassium nutrition of mycorrhizal pine plants was significantly improved under potassium-limiting conditions. Fungal strains overexpressing HcTrk1 reduced the translocation of potassium and phosphorus from the roots to the shoots of inoculated plants in mycorrhizal experiments. Furthermore, expression of HcTrk1 and the phosphate transporter HcPT1.1 were reciprocally linked to the external inorganic phosphate and potassium availability. The development of these approaches provides a deeper insight into the role of ectomycorrhizal symbiosis on host plant K(+) nutrition and in particular, the K(+) transporter HcTrk1. The work augments our knowledge of the link between potassium and phosphorus nutrition via the mycorrhizal pathway.<br /> (© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.)
- Subjects :
- Biological Transport drug effects
DNA, Bacterial genetics
Gene Expression Regulation, Fungal drug effects
Hebeloma drug effects
Hebeloma genetics
Hyphae drug effects
Hyphae metabolism
Mycorrhizae drug effects
Mycorrhizae genetics
Phenotype
Phosphorus pharmacology
Pinus drug effects
Pinus microbiology
Potassium pharmacology
RNA, Messenger genetics
RNA, Messenger metabolism
Seedlings drug effects
Seedlings metabolism
Seedlings microbiology
Sodium metabolism
Fungal Proteins metabolism
Hebeloma metabolism
Host-Pathogen Interactions drug effects
Mycorrhizae metabolism
Phosphorus metabolism
Pinus metabolism
Potassium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 201
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 24279702
- Full Text :
- https://doi.org/10.1111/nph.12603