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Contribution of molecular diagnosis to the management of cutaneous leishmaniasis in travellers.
- Source :
-
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2014 Aug; Vol. 20 (8), pp. O528-30. Date of Electronic Publication: 2014 Jan 08. - Publication Year :
- 2014
-
Abstract
- Cutaneous leishmaniasis is one of the most frequent skin diseases occurring after travelling in endemic areas. Optimal management requires identification of the species of Leishmania involved. In this study we aimed to evaluate the use of molecular diagnosis as routine, in comparison with direct examination and culture. Thirty positive diagnoses were carried out between 2007 and 2013. Classical PCR enabled 11 positive cases to be identified that were found to be negative by conventional methods. Sequencing led to the identification of eight different species. Routine use of PCR and sequencing appears very efficient in the management of cutaneous leishmaniasis.<br /> (© 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Child
Child, Preschool
Female
Humans
Leishmania classification
Leishmania genetics
Male
Middle Aged
Polymerase Chain Reaction methods
Sensitivity and Specificity
Sequence Analysis, DNA methods
Young Adult
Leishmania isolation & purification
Leishmaniasis, Cutaneous diagnosis
Molecular Diagnostic Techniques methods
Travel
Subjects
Details
- Language :
- English
- ISSN :
- 1469-0691
- Volume :
- 20
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 24279601
- Full Text :
- https://doi.org/10.1111/1469-0691.12487