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Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.

Authors :
Mariashibu TS
Subramanyam K
Arun M
Theboral J
Rajesh M
Rengan SK
Chakravarthy R
Manickavasagam M
Ganapathi A
Source :
Indian journal of experimental biology [Indian J Exp Biol] 2013 Oct; Vol. 51 (10), pp. 849-59.
Publication Year :
2013

Abstract

Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 microM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 microM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 microM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.

Details

Language :
English
ISSN :
0019-5189
Volume :
51
Issue :
10
Database :
MEDLINE
Journal :
Indian journal of experimental biology
Publication Type :
Academic Journal
Accession number :
24266110