Back to Search
Start Over
Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
- Source :
-
Indian journal of experimental biology [Indian J Exp Biol] 2013 Oct; Vol. 51 (10), pp. 849-59. - Publication Year :
- 2013
-
Abstract
- Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 microM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 microM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 microM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
- Subjects :
- Acclimatization drug effects
Acclimatization physiology
Amino Acids pharmacology
Cell Differentiation drug effects
Cell Proliferation drug effects
Cotyledon drug effects
Cotyledon growth & development
Cotyledon physiology
Desiccation
Germination drug effects
Germination physiology
Plant Growth Regulators pharmacology
Glycine max drug effects
Glycine max growth & development
Glycine max physiology
Plant Somatic Embryogenesis Techniques methods
Glycine max embryology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-5189
- Volume :
- 51
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Indian journal of experimental biology
- Publication Type :
- Academic Journal
- Accession number :
- 24266110