Back to Search
Start Over
Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining.
- Source :
-
Comparative cytogenetics [Comp Cytogenet] 2012 Apr 27; Vol. 6 (2), pp. 183-97. Date of Electronic Publication: 2012 Apr 27 (Print Publication: 2012). - Publication Year :
- 2012
-
Abstract
- The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38-46), Dicerca aenea validiuscula Semenov, 1895 - 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 - 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining.
Details
- Language :
- English
- ISSN :
- 1993-0771
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comparative cytogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 24260661
- Full Text :
- https://doi.org/10.3897/CompCytogen.v6i2.2950