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Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets.
- Source :
-
Veterinary research communications [Vet Res Commun] 2014 Mar; Vol. 38 (1), pp. 39-49. Date of Electronic Publication: 2013 Nov 19. - Publication Year :
- 2014
-
Abstract
- The use of transgenic plants as delivery system for antigenic proteins is attractive for its simplicity and increases likelihood for local immune response at sites of infection. The aim of this study was to evaluate the protective effect of oral administration of tobacco seeds, expressing the FedA, the major protein of the F18 adhesive fimbriae, and B subunit of verocytotoxin, against verocytotoxin-producing E. coli (VTEC) strain in piglets. Forty-three early weaned piglets, were randomly divided into 4 experimental groups: 3 test groups and a control. Treatment groups orally received a bolus, with different dose of tobacco seeds on 0, 1, 2, 14 days post primary administration. After challenge, with 1*10(10) CFU of O138 Escherichia coli strain, piglets showed clinical scores significantly higher in the control group compared to orally immunized groups (Pā<ā0.05) and the latter showed a faster recovery than in CG. In conclusion, oral administration of recombinant tobacco seeds expressing antigenic proteins against VTEC strains can induce a protective effect against challenger strain in piglets.
- Subjects :
- Administration, Oral
Animals
Antibodies, Bacterial analysis
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Bacterial Vaccines standards
Escherichia coli Infections prevention & control
Escherichia coli Proteins genetics
Feces chemistry
Fimbriae Proteins genetics
Immunization methods
Immunoglobulin A analysis
Plants, Genetically Modified genetics
Plants, Genetically Modified immunology
Random Allocation
Seeds genetics
Shiga-Toxigenic Escherichia coli
Swine
Nicotiana immunology
Bacterial Vaccines administration & dosage
Escherichia coli Infections veterinary
Immunization veterinary
Seeds immunology
Swine Diseases prevention & control
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-7446
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary research communications
- Publication Type :
- Academic Journal
- Accession number :
- 24249478
- Full Text :
- https://doi.org/10.1007/s11259-013-9583-9