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Evaluation of the comet assay for assessing the dose-response relationship of DNA damage induced by ionizing radiation.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2013 Nov 14; Vol. 14 (11), pp. 22449-61. Date of Electronic Publication: 2013 Nov 14. - Publication Year :
- 2013
-
Abstract
- Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 14
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 24240807
- Full Text :
- https://doi.org/10.3390/ijms141122449