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"Candidatus Sonnebornia yantaiensis", a member of candidate division OD1, as intracellular bacteria of the ciliated protist Paramecium bursaria (Ciliophora, Oligohymenophorea).
- Source :
-
Systematic and applied microbiology [Syst Appl Microbiol] 2014 Feb; Vol. 37 (1), pp. 35-41. Date of Electronic Publication: 2013 Nov 11. - Publication Year :
- 2014
-
Abstract
- An intracellular bacterium was discovered in an isolate of Paramecium bursaria from a freshwater pond in Yantai, China. The bacteria were abundant and exclusively found in the cytoplasm of the host which, along with the green alga Chlorella, formed a three-partner consortium that could survive in pure water for at least one week. Cloning, sequencing and phylogenetic analysis of the bacterial 16S rRNA gene showed that the bacterium belonged to the uncultured candidate division OD1, which usually forms part of the rare biosphere. Transmission electron microscopy and fluorescence in situ hybridization (FISH) with specific probes showed that the bacteria were usually located close to the perialgal membranes of endosymbiotic Chlorella cells, and occasionally irregularly distributed throughout the host cytoplasm. The name "Candidatus Sonnebornia yantaiensis" gen. nov., sp. nov. is proposed for the new bacterium. A strongly supported monophyletic subclade, OD1-p, which included the new species, was recognized and this study highlights that protists can be important hosts for rare bacterial taxa.<br /> (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Subjects :
- Bacteria genetics
Bacteria ultrastructure
China
Cluster Analysis
Cytoplasm microbiology
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fresh Water
In Situ Hybridization, Fluorescence
Microbial Consortia
Microscopy, Electron, Transmission
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Bacteria classification
Bacteria isolation & purification
Paramecium microbiology
Ponds microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0984
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Systematic and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 24231291
- Full Text :
- https://doi.org/10.1016/j.syapm.2013.08.007