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EBP1, a novel host factor involved in primer binding site-dependent restriction of moloney murine leukemia virus in embryonic cells.
- Source :
-
Journal of virology [J Virol] 2014 Feb; Vol. 88 (3), pp. 1825-9. Date of Electronic Publication: 2013 Nov 13. - Publication Year :
- 2014
-
Abstract
- Mouse embryonic cells are unable to support the replication of Moloney murine leukemia virus (MLV). The integrated viral DNA is transcriptionally silenced, largely due to binding of host transcriptional repressors to the primer binding site (PBS) of the provirus. We have previously shown that a PBS DNA-binding repressor complex contains ZFP809 and TRIM28. Here, we identified ErbB3-binding protein 1 (EBP1) to be a novel component of the ZFP809-TRIM28 silencing complex and show that EBP1 depletion reduces PBS-mediated retroviral silencing.
- Subjects :
- Animals
Binding Sites
Cell Line, Tumor
DNA Primers metabolism
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Leukemia embryology
Leukemia genetics
Leukemia metabolism
Leukemia virology
Mice
Moloney murine leukemia virus chemistry
Moloney murine leukemia virus physiology
Nuclear Proteins genetics
Protein Binding
RNA, Viral chemistry
RNA, Viral genetics
RNA, Viral metabolism
RNA-Binding Proteins
Repressor Proteins genetics
Repressor Proteins metabolism
Rodent Diseases embryology
Rodent Diseases genetics
Rodent Diseases virology
Tripartite Motif-Containing Protein 28
Virus Replication
DNA Primers genetics
Gene Silencing
Leukemia veterinary
Moloney murine leukemia virus genetics
Nuclear Proteins metabolism
Rodent Diseases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 88
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 24227866
- Full Text :
- https://doi.org/10.1128/JVI.02578-13