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Calcium increases the yield of somatic embryos in carrot embryogenic suspension cultures.
- Source :
-
Plant cell reports [Plant Cell Rep] 1990 Aug; Vol. 9 (4), pp. 221-3. - Publication Year :
- 1990
-
Abstract
- An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10(-3) M were transferred to hormone-free medium containing 10(-2) M calcium and when embryogenic suspension cells grown in 2,4-D medium containing 10(-4) M calcium were transferred to hormone-free medium with 10(-3) M calcium. At calcium concentrations between 6·10(-3) and 10(-2) M globular stage somatic embryos were found in cultures supplemented with 2·10(-6) M of 2,4-D indicating that elevated calcium counteracts the inhibitory effect of 2,4-D on somatic embryogenesis. No qualitative changes were found in the pattern of extracellular polypeptides as a result of growth and embryogenesis in media with different calcium concentrations.
Details
- Language :
- English
- ISSN :
- 0721-7714
- Volume :
- 9
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 24226707
- Full Text :
- https://doi.org/10.1007/BF00232184