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Desmoglein-1 regulates esophageal epithelial barrier function and immune responses in eosinophilic esophagitis.

Desmoglein-1 regulates esophageal epithelial barrier function and immune responses in eosinophilic esophagitis.

Authors :
Sherrill JD
Kc K
Wu D
Djukic Z
Caldwell JM
Stucke EM
Kemme KA
Costello MS
Mingler MK
Blanchard C
Collins MH
Abonia JP
Putnam PE
Dellon ES
Orlando RC
Hogan SP
Rothenberg ME
Source :
Mucosal immunology [Mucosal Immunol] 2014 May; Vol. 7 (3), pp. 718-29. Date of Electronic Publication: 2013 Nov 13.
Publication Year :
2014

Abstract

The desmosomal cadherin desmoglein-1 (DSG1) is an essential intercellular adhesion molecule that is altered in various human cutaneous disorders; however, its regulation and function in allergic disease remains unexplored. Herein, we demonstrate a specific reduction in DSG1 in esophageal biopsies from patients with eosinophilic esophagitis (EoE), an emerging allergic disorder characterized by chronic inflammation within the esophageal mucosa. Further, we show that DSG1 gene silencing weakens esophageal epithelial integrity, and induces cell separation and impaired barrier function (IBF) despite high levels of desmoglein-3. Moreover, DSG1 deficiency induces transcriptional changes that partially overlap with the transcriptome of inflamed esophageal mucosa; notably, periostin (POSTN), a multipotent pro-inflammatory extracellular matrix molecule, is the top induced overlapping gene. We further demonstrate that IBF is a pathological feature in EoE, which can be partially induced through the downregulation of DSG1 by interleukin-13 (IL-13). Taken together, these data identify a functional role for DSG1 and its dysregulation by IL-13 in the pathophysiology of EoE and suggest that the loss of DSG1 may potentiate allergic inflammation through the induction of pro-inflammatory mediators such as POSTN.

Details

Language :
English
ISSN :
1935-3456
Volume :
7
Issue :
3
Database :
MEDLINE
Journal :
Mucosal immunology
Publication Type :
Academic Journal
Accession number :
24220297
Full Text :
https://doi.org/10.1038/mi.2013.90