Alpha-lipoic acid protects cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy.

Authors :: Cao X
Chen A
Yang P
Song X
Liu Y
Li Z
Wang X
Wang L
Li Y
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Nov 29; Vol. 441 (4), pp. 935-40. Date of Electronic Publication: 2013 Nov 09.
Publication Year :: 2013
Abstract: Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Alpha-lipoic acid (LA) plays an important role in the etiology of cardiovascular disease. Autophagy is widely implicated in myocardial I/R injury. We assessed the degree of autophagy by pretreatment with LA exposed to H/R in H9c2 cell based on the expression levels of Beclin-1, LC3II/LC3I, and green fluorescent protein-labeled LC3 fusion proteins. Autophagic vacuoles were confirmed in H9c2 cells exposed to H/R using transmission electron microscopy. Our findings indicated that pretreatment with LA inhibited the degree of autophagy in parallel to the enhanced cell survival and decreased total cell death in H9c2 cells exposed to H/R. We conclude that LA protects cardiomyocytes against H/R injury by inhibiting autophagy.<br /> (Copyright © 2013. Published by Elsevier Inc.)

Subjects :: Animals
Apoptosis drug effects
Apoptosis Regulatory Proteins metabolism
Beclin-1
Cells, Cultured
Green Fluorescent Proteins metabolism
Microtubule-Associated Proteins metabolism
Myocytes, Cardiac ultrastructure
Recombinant Fusion Proteins metabolism
Vacuoles metabolism
Vacuoles ultrastructure
Autophagy drug effects
Myocardial Reperfusion Injury prevention & control
Myocytes, Cardiac drug effects
Protective Agents pharmacology
Thioctic Acid pharmacology

Full Text Access

Tools