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Effect of the concentration of phenothiazine photosensitizers in antimicrobial photodynamic therapy on bone loss and the immune inflammatory response of induced periodontitis in rats.
- Source :
-
Journal of periodontal research [J Periodontal Res] 2014 Oct; Vol. 49 (5), pp. 584-94. Date of Electronic Publication: 2013 Nov 09. - Publication Year :
- 2014
-
Abstract
- Background and Objective: Antimicrobial therapy can suppress periodontal pathogens and increase the effectiveness of conventional mechanical treatment. The aim of this study was to assess bone loss and the immune inflammatory response of rats under the influence of two photosensitizing agents (MB and TBO) at two different concentrations in antimicrobial photodynamic therapy (aPDT), used as an adjuvant therapy in the treatment of periodontitis.<br />Material and Methods: Periodontitis was induced in the mandibular first molars of 162 rats. The animals were divided into nine groups: G1 - scaling and root planing (SRP); G2 - SRP plus 100 μg/mL of methylene blue (MB); G3 - SRP plus 10 mg/mL of MB; G4 - SRP plus 100 μg/mL of toluidine blue (TBO); G5 - SRP plus 10 mg/mL of TBO; G6 - SRP plus 100 μg/mL of MB and laser; G7 - SRP plus 10 mg/mL of MB and laser; G8 - SRP plus 100 μg/mL of TBO and laser; and G9 - SRP plus 10 mg/mL of TBO and laser. Six animals from each group were euthanized 7, 15, or 30 d after treatment. Bone loss (BL) in the furcation region was evaluated using histomorphometric and immunohistochemical analyses to detect the receptor activator of nuclear factor-Κappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP).<br />Results: There was significantly less BL in animals treated with aPDT using low concentrations of MB and TBO at 7, 15 and 30 d. Immunohistochemical analysis revealed decreased RANKL and increased OPG in the aPDT groups and decreased TRAP-positive cells in G6 and G8.<br />Conclusions: aPDT, using low concentrations of MB and TBO, was the most effective adjuvant therapy to SRP, acting indirectly as a downregulator of the molecular mechanisms that control bone resorption in periodontitis.<br /> (© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Acid Phosphatase analysis
Alveolar Bone Loss immunology
Animals
Combined Modality Therapy
Connective Tissue pathology
Dental Scaling methods
Isoenzymes analysis
Low-Level Light Therapy instrumentation
Lymphocytes pathology
Male
Methylene Blue administration & dosage
Neutrophils pathology
Osteoclasts pathology
Osteoprotegerin analysis
Periodontitis immunology
RANK Ligand analysis
Rats
Rats, Wistar
Root Planing methods
Tartrate-Resistant Acid Phosphatase
Time Factors
Tolonium Chloride administration & dosage
Alveolar Bone Loss drug therapy
Periodontitis drug therapy
Phenothiazines administration & dosage
Photochemotherapy methods
Photosensitizing Agents administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0765
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of periodontal research
- Publication Type :
- Academic Journal
- Accession number :
- 24206053
- Full Text :
- https://doi.org/10.1111/jre.12138