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[Cytochrome oxidase I gene sequences that cause hypodermosis cattle species by PCR-RFLP technique investigation].
- Source :
-
Turkiye parazitolojii dergisi [Turkiye Parazitol Derg] 2013; Vol. 37 (3), pp. 190-4. - Publication Year :
- 2013
-
Abstract
- Objective: The importance for the health of cattle Hypodermosis (Nokra) species that cause disease by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method was investigated.<br />Methods: The molecular analysis of Hypoderma species specific sequences located on the Cytochrome C Oxidase I (COI) (mtDNA) gene by PCR-RFLP method was successfully carried out.<br />Results: DNAs were obtained using the extraction kit and Polymerase chain reaction (PCR) foe amplification of the mitochondrial DNA gene yielded for 688bp COI gene on agarose gel. Using the PCR amplification and HinfI, RsaI and TaqI restriction enzymes, mitochondrial DNA gene regions were amplified with the RFLP technique.<br />Conclusion: The mitochondrial COI gene region of the genus Hypoderma for the molecular identification of potential answers questions, such as the level, which indicates that it may be very useful. In this study, in the city of Van, it is also important that the Hypoderma species be defined in terms of the first molecular study.
- Subjects :
- Animals
Base Sequence
Cattle
Cattle Diseases parasitology
DNA, Mitochondrial genetics
Hypodermyiasis parasitology
Hypodermyiasis veterinary
Mitochondria genetics
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Diptera genetics
Electron Transport Complex IV genetics
Genes, Insect
Subjects
Details
- Language :
- Turkish
- ISSN :
- 2146-3077
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Turkiye parazitolojii dergisi
- Publication Type :
- Academic Journal
- Accession number :
- 24192621
- Full Text :
- https://doi.org/10.5152/tpd.2013.42