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Ketone bodies promote a rapid rise in glutamate efflux from the isolated perfused rat liver without altering the rate of glutamine production.

Authors :
Almond MK
Smith A
Cohen RD
Source :
Amino acids [Amino Acids] 1995 Jun; Vol. 9 (2), pp. 141-6.
Publication Year :
1995

Abstract

Livers of starved (48 hr) male Wistar rats were perfused in a non recirculating manner with a near physiological mix of ammonium, lactate, ornithine and pyruvate in Krebs buffer. The addition of ketone bodies (3-DL-hydroxybutyrate [B OHB] 2-30 mM or lithium-acetoacetate (15 mM) to the perfusate resulted in a rapid rise in the efflux of glutamate from the liver (five times above basal). This was not seen with control solutions (sodium chloride or lithium chloride). The increased efflux was sustained for the duration of the addition of the ketone bodies (7 min), was rapidly reversible and dose dependant. Glutamine export rates were not altered, suggesting that either the glutamate originated from cells not responsible for glutamine synthesis or that this glutamate was superfulous to the requirement of glutamine synthesis. There was no evidence that the lactate transporter was involved in the entry of lactate into perivenous hepatocytes for glutamine synthesis; lactate presumably entering the hepatocyte by an alternative pathway, probably nonionic diffusion.

Details

Language :
English
ISSN :
0939-4451
Volume :
9
Issue :
2
Database :
MEDLINE
Journal :
Amino acids
Publication Type :
Academic Journal
Accession number :
24178814
Full Text :
https://doi.org/10.1007/BF00805835