Back to Search
Start Over
Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2014 Jan; Vol. 9 (1), pp. 185-91. Date of Electronic Publication: 2013 Oct 25. - Publication Year :
- 2014
-
Abstract
- The purpose of this study was to investigate the isolation and culture of muscle‑derived stem cells (MDSCs) and their capability to differentiate into osteoblasts in vitro. Skeletal muscle tissue was obtained from double hind limbs of New Zealand white rabbits under sterile conditions and isolated by collagenase digestion. Following passages in basic medium, the primary cells were desmin (+), myosin (+) and CD105 (+). Differentiation of MDSCs was induced by osteogenic medium. Using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide assay, the differentiated cell population was found to proliferate faster than the undifferentiated. Alkaline phosphatase staining and alizarin red staining revealed that the differentiated cells were mineralized in vitro. Quantitative polymerase chain reaction assays also showed increased mRNA expression of osteogenic genes in differentiated cells. In conclusion, stem cells were successfully isolated and cultured from rabbit skeletal muscle tissue and were able to differentiate into osteoblasts following induction. These observations may indicate an ideal stem cell source for tissue engineering.
- Subjects :
- Animals
Bone and Bones cytology
Cell Differentiation
Mesenchymal Stem Cells metabolism
Osteocalcin genetics
Osteocalcin metabolism
Osteopontin genetics
Osteopontin metabolism
RNA, Messenger metabolism
Rabbits
Mesenchymal Stem Cells cytology
Muscle, Skeletal cytology
Osteogenesis
Tissue Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 24173582
- Full Text :
- https://doi.org/10.3892/mmr.2013.1758