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RNA Interference Targeting Snail Inhibits the Transforming Growth Factor β 2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells.
- Source :
-
Journal of ophthalmology [J Ophthalmol] 2013; Vol. 2013, pp. 869101. Date of Electronic Publication: 2013 Sep 14. - Publication Year :
- 2013
-
Abstract
- Epithelial-msenchymal transition (EMT) contributes to posterior capsule opacification (PCO) type of cataract. Transcription factors Snail is a key trigger of EMT activated by transforming growth factor β (TGF β ). This study was done to investigate the effect of Snail targeting siRNA on TGF β 2-induced EMT in human lens epithelial cells. TGF β 2 treatment of cultured human epithelial cell line (HLEB3) upregulated the expression of Snail and the EMT relevant molecules such as vimentin and α -SMA but downregulated the expression of keratin and E-cadherin. After the stimulation of TGF β 2, the HLEB3 cells became fibroblast-like in morphology, and the junctions of cell-cell disappeared. TGF β 2 treatment also enhanced migration ability of HLEB3 cells. TGF β 2-induced Snail expression and EMT were significantly inhibited by Snail siRNA. By analyzing the response characteristics of HLEB3 in TGF β 2-induced EMT model with/without Snail-specific siRNA, we concluded that Snail is an element in the EMT of HLEB3 cells induced by TGF β 2. Snail siRNA targeting can block the induced EMT and therefore has the potential to suppress the development of PCO.
Details
- Language :
- English
- ISSN :
- 2090-004X
- Volume :
- 2013
- Database :
- MEDLINE
- Journal :
- Journal of ophthalmology
- Publication Type :
- Academic Journal
- Accession number :
- 24163761
- Full Text :
- https://doi.org/10.1155/2013/869101