Back to Search
Start Over
Amplification of steroid-mediated SP-B expression by physiological levels of caffeine.
- Source :
-
American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2014 Jan 01; Vol. 306 (1), pp. L101-9. Date of Electronic Publication: 2013 Oct 25. - Publication Year :
- 2014
-
Abstract
- Factors positively influencing surfactant homeostasis in general and surfactant protein B (SP-B) expression in particular are considered of clinical importance regarding an improvement of lung function in preterm infants. The objective of this study was to identify effects of physiological levels of caffeine on glucocorticoid-mediated SP-B expression in vitro and in vivo. Levels of SP-B and pepsinogen C were quantified by quantitative real-time RT-PCR or immunoblotting in NCI-H441 cells daily exposed to caffeine and/or dexamethasone (DEX). In vivo, SP-B expression was analyzed in bronchoalveolar lavage (BAL) of preterm sheep exposed to antenatal DEX and/or postnatal caffeine. If DEX and caffeine were continuously present, SP-B mRNA and protein levels were increased for up to 6 days after induction (P < 0.05). Additionally, caffeine enhanced SP-B mRNA expression in DEX-pretreated cells (P < 0.05). Moreover, caffeine amplified DEX-induced pepsinogen C mRNA expression (P < 0.05). After short-term treatment with caffeine in vivo, only slightly higher SP-B levels could be detected in BAL of preterm sheep following antenatal DEX, combined with an increase of arterial oxygen partial pressure (P < 0.01). Our data demonstrated that the continuous presence of caffeine in vitro is able to amplify DEX-mediated SP-B expression. In contrast, short-term improvement of lung function in vivo is likely to be independent of altered SP-B transcription and translation. An impact of caffeine on release of surfactant reservoirs from lamellar bodies could, however, quickly affect SP-B content in BAL, which has to be further investigated. Our findings indicate that caffeine is able to amplify main effects of glucocorticoids that result from changes in surfactant production, maturation, and release.
- Subjects :
- Animals
Antigens, Surface genetics
Antigens, Surface metabolism
Bronchoalveolar Lavage Fluid
Cathepsin H genetics
Cathepsin H metabolism
Female
Fetus drug effects
Fetus metabolism
Glucocorticoids physiology
Humans
Lung drug effects
Lung metabolism
Maternal-Fetal Exchange
Oxygen blood
Pepsinogen C genetics
Pepsinogen C metabolism
Pregnancy
Pulmonary Surfactant-Associated Protein B genetics
Sheep
Caffeine pharmacology
Dexamethasone pharmacology
Gene Expression
Glucocorticoids pharmacology
Pulmonary Surfactant-Associated Protein B metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1504
- Volume :
- 306
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Lung cellular and molecular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 24163141
- Full Text :
- https://doi.org/10.1152/ajplung.00257.2013