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Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells.
- Source :
-
Acta biomaterialia [Acta Biomater] 2014 Jan; Vol. 10 (1), pp. 428-38. Date of Electronic Publication: 2013 Oct 21. - Publication Year :
- 2014
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Abstract
- The nanostructured surface of biomaterials plays an important role in improving their in vitro cellular bioactivity as well as stimulating in vivo tissue regeneration. Inspired by the mussel's adhesive versatility, which is thought to be due to the plaque-substrate interface being rich in 3,4-dihydroxy-l-phenylalamine (DOPA) and lysine amino acids, in this study we developed a self-assembly method to prepare a uniform calcium phosphate (Ca-P)/polydopamine composite nanolayer on the surface of β-tricalcium phosphate (β-TCP) bioceramics by soaking β-TCP bioceramics in Tris-dopamine solution. It was found that the addition of dopamine, reaction temperature and reaction time are three key factors inducing the formation of a uniform Ca-P/polydopamine composite nanolayer. The formation mechanism of a Ca-P/polydopamine composite nanolayer involved two important steps: (i) the addition of dopamine to Tris-HCl solution decreases the pH value and accelerates Ca and P ionic dissolution from the crystal boundaries of β-TCP ceramics; (ii) dopamine is polymerized to form self-assembled polydopamine film and, at the same time, nanosized Ca-P particles are mineralized with the assistance of polydopamine, in which the formation of polydopamine occurs simultaneously with Ca-P mineralization (formation of nanosized microparticles composed of calcium phosphate-based materials), and finally a self-assembled Ca-P/polydopamine composite nanolayer forms on the surface of the β-TCP ceramics. Furthermore, the formed self-assembled Ca-P/polydopamine composite nanolayer significantly enhances the surface roughness and hydrophilicity of β-TCP ceramics, and stimulates the attachment, proliferation, alkaline phosphate (ALP) activity and bone-related gene expression (ALP, OCN, COL1 and Runx2) of human bone marrow stromal cells. Our results suggest that the preparation of self-assembled Ca-P/polydopamine composite nanolayers is a viable method to modify the surface of biomaterials by significantly improving their surface physicochemical properties and cellular bioactivity for bone regeneration application.<br /> (Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Alkaline Phosphatase metabolism
Animals
Bone Marrow Cells drug effects
Bone Marrow Cells metabolism
Bone Marrow Cells ultrastructure
Calcium Phosphates pharmacology
Cell Differentiation drug effects
Cell Differentiation genetics
Cell Proliferation drug effects
Ceramics chemical synthesis
Gene Expression Regulation drug effects
Humans
Hydrogen-Ion Concentration drug effects
Hydrophobic and Hydrophilic Interactions drug effects
Microscopy, Atomic Force
Nanoparticles ultrastructure
Osteogenesis genetics
Stromal Cells cytology
Stromal Cells drug effects
Stromal Cells metabolism
Stromal Cells ultrastructure
Surface Properties
Temperature
Time Factors
Water chemistry
Biocompatible Materials pharmacology
Bivalvia chemistry
Bone Marrow Cells cytology
Ceramics pharmacology
Indoles pharmacology
Nanoparticles chemistry
Osteogenesis drug effects
Polymers pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-7568
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Acta biomaterialia
- Publication Type :
- Academic Journal
- Accession number :
- 24157695
- Full Text :
- https://doi.org/10.1016/j.actbio.2013.10.013