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Forskolin induces supersensitivity of the amylase secretory response of rat parotid tissue.
- Source :
-
Japanese journal of pharmacology [Jpn J Pharmacol] 1985 Sep; Vol. 39 (1), pp. 39-44. - Publication Year :
- 1985
-
Abstract
- The stimulatory effect of forskolin on amylase secretion was studied by pretreating rat parotid tissue with forskolin for 10 min, incubating it in medium without forskolin for 10 min, and then treating it with forskolin again. Pretreatment with 10 microM forskolin for 10 min resulted in increased amylase secretion and enhanced accumulation of cyclic AMP in the tissue during the second incubation with forskolin. In the presence of colchicine or vinblastine, the enhancement in cyclic AMP accumulation during the second incubation with forskolin was prevented, but the increased amylase secretion remained unchanged. The increased amylase secretion was counteracted only in the presence of concanavalin A. On the other hand, increased amylase secretion induced by isoproterenol (IPR) pretreatment was counteracted by colchicine, vinblastine, concanavalin A or strychnine. These data suggest that the total amount of cyclic AMP in the tissue does not have any essential role in the supersensitivity of the amylase secretory response, and that the supersensitivity induced by forskolin differs from that induced by IPR.
- Subjects :
- Animals
Colchicine pharmacology
Concanavalin A pharmacology
Cyclic AMP metabolism
In Vitro Techniques
Isoproterenol pharmacology
Male
Parotid Gland metabolism
Rats
Rats, Inbred Strains
Strychnine pharmacology
Vinblastine pharmacology
Amylases metabolism
Colforsin pharmacology
Parotid Gland enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-5198
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Japanese journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 2415738
- Full Text :
- https://doi.org/10.1254/jjp.39.39