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The adenovirus 55 residue E1A protein is a transcriptional activator and binds the unliganded thyroid hormone receptor.
- Source :
-
The Journal of general virology [J Gen Virol] 2014 Jan; Vol. 95 (Pt 1), pp. 142-152. Date of Electronic Publication: 2013 Oct 17. - Publication Year :
- 2014
-
Abstract
- The early region 1A (E1A) of human adenovirus types 2 and 5 is differentially spliced to yield five distinct mRNAs that encode different proteins. The smallest E1A RNA transcript encodes a 55 residue (55R) protein that shares only 28 amino acid residues with the other E1A proteins. Even though it is the most abundant E1A transcript at late times post-infection, little is known about the functions of this E1A isoform. In this study, we show that the E1A 55R protein interacts with, and modulates the activity of the unliganded thyroid hormone receptor (TR). We demonstrate that E1A 55R contains a signature motif known as the CoRNR box that confers interaction with the unliganded TR; this motif was originally identified in cellular corepressors. Using a system reconstituted in the yeast Saccharomyces cerevisiae, which lack endogenous TR and TR coregulators, we show that E1A 55R nonetheless differs from cellular corepressors as it functions as a strong co-activator of TR-dependent transcription and that it possesses an intrinsic transcriptional activation domain. These data indicate that the E1A 55R protein functions as a transcriptional regulator.
- Subjects :
- Adenovirus E1A Proteins genetics
Adenovirus Infections, Human metabolism
Adenovirus Infections, Human virology
Adenoviruses, Human chemistry
Adenoviruses, Human genetics
Cell Line, Tumor
Gene Expression Regulation
Humans
Protein Binding
Protein Structure, Tertiary
Trans-Activators chemistry
Trans-Activators genetics
Transcriptional Activation
Adenovirus E1A Proteins chemistry
Adenovirus E1A Proteins metabolism
Adenovirus Infections, Human genetics
Adenoviruses, Human metabolism
Promoter Regions, Genetic
Receptors, Thyroid Hormone genetics
Trans-Activators metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2099
- Volume :
- 95
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 24136366
- Full Text :
- https://doi.org/10.1099/vir.0.056838-0