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The Tomato 14-3-3 protein TFT4 modulates H+ efflux, basipetal auxin transport, and the PKS5-J3 pathway in the root growth response to alkaline stress.
- Source :
-
Plant physiology [Plant Physiol] 2013 Dec; Vol. 163 (4), pp. 1817-28. Date of Electronic Publication: 2013 Oct 17. - Publication Year :
- 2013
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Abstract
- Alkaline stress is a common environmental stress, in particular in salinized soils. Plant roots respond to a variety of soil stresses by regulating their growth, but the nature of the regulatory pathways engaged in the alkaline stress response (ASR) is not yet understood. Previous studies show that PIN-FORMED2, an auxin (indole-3-acetic acid [IAA]) efflux transporter, PKS5, a protein kinase, and DNAJ HOMOLOG3 (J3), a chaperone, play key roles in root H(+) secretion by regulating plasma membrane (PM) H(+)-ATPases directly or by targeting 14-3-3 proteins. Here, we investigated the expression of all 14-3-3 gene family members (TOMATO 14-3-3 PROTEIN1 [TFT1]-TFT12) in tomato (Solanum lycopersicum) under ASR, showing the involvement of four of them, TFT1, TFT4, TFT6, and TFT7. When these genes were separately introduced into Arabidopsis (Arabidopsis thaliana) and overexpressed, only the growth of TFT4 overexpressors was significantly enhanced when compared with the wild type under stress. H(+) efflux and the activity of PM H(+)-ATPase were significantly enhanced in the root tips of TFT4 overexpressors. Microarray analysis and pharmacological examination of the overexpressor and mutant plants revealed that overexpression of TFT4 maintains primary root elongation by modulating PM H(+)-ATPase-mediated H(+) efflux and basipetal IAA transport in root tips under alkaline stress. TFT4 further plays important roles in the PKS5-J3 signaling pathway. Our study demonstrates that TFT4 acts as a regulator in the integration of H(+) efflux, basipetal IAA transport, and the PKS5-J3 pathway in the ASR of roots and coordinates root apex responses to alkaline stress for the maintenance of primary root elongation.
- Subjects :
- 14-3-3 Proteins genetics
Alkalies pharmacology
Arabidopsis drug effects
Arabidopsis genetics
Biological Transport drug effects
Biomass
Cell Membrane drug effects
Cell Membrane enzymology
Gene Expression Profiling
Gene Expression Regulation, Plant drug effects
Genes, Plant genetics
Solanum lycopersicum drug effects
Solanum lycopersicum genetics
Meristem drug effects
Meristem enzymology
Multigene Family
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots drug effects
Plant Roots metabolism
Plants, Genetically Modified
Proton-Translocating ATPases metabolism
14-3-3 Proteins metabolism
Indoleacetic Acids metabolism
Solanum lycopersicum physiology
Plant Roots growth & development
Protons
Signal Transduction drug effects
Signal Transduction genetics
Stress, Physiological drug effects
Stress, Physiological genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 163
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 24134886
- Full Text :
- https://doi.org/10.1104/pp.113.224758