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Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation.
- Source :
-
Biotechnology progress [Biotechnol Prog] 2013 Sep-Oct; Vol. 29 (5), pp. 1116-23. Date of Electronic Publication: 2013 Sep 20. - Publication Year :
- 2013
-
Abstract
- A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40°C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30°C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.<br /> (© 2013 American Institute of Chemical Engineers.)
- Subjects :
- Gas Chromatography-Mass Spectrometry
Heat-Shock Proteins genetics
Heat-Shock Proteins metabolism
Hot Temperature
Metabolomics
Oligonucleotide Array Sequence Analysis
RNA, Fungal genetics
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Trehalose metabolism
Adaptation, Physiological genetics
Gene Expression Regulation, Fungal
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6033
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biotechnology progress
- Publication Type :
- Academic Journal
- Accession number :
- 24115578
- Full Text :
- https://doi.org/10.1002/btpr.1754