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Development of a paper-based carbon nanotube sensing microfluidic device for biological detection.
- Source :
-
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference [Annu Int Conf IEEE Eng Med Biol Soc] 2013; Vol. 2013, pp. 168-71. - Publication Year :
- 2013
-
Abstract
- Carbon nanotube (CNT) has been utilized for the biological detection due to its extremely sensitive to biological molecules. A paper-based CNT sensing microfluidic device has been developed for the detection of protein, i.e., biotin-avidin, binding. We have developed a fabrication method that allows controlled deposition of bundled CNTs with well-defined dimensions to form sensors on paper. Then, polydimethyl siloxane (PDMS) was used to pattern the hydrophobic boundary on paper to form the reaction sites. The proposed fabrication method is based on vacuum filtration process with a metal mask covering on a filter paper for the definition of the dimension of sensor. The length, width, and thickness of the CNT-based sensors are readily controlled by the metal mask and the weight of the CNT powder used during the filtration process, respectively. Homogeneous deposition of CNTs with well-defined dimensions can be achieved. The CNT-based sensor on paper has been demonstrated on the detection of the protein binding. Biotin was first immobilized on the CNT's sidewall and avidin suspended solution was applied to the site. The result of the biotin-avidin binding was measured by the resistance change of the sensor, which is a label-free detection method. It showed the CNT is sensitive to the biological molecules and the proposed paper-based CNT sensing device is a possible candidate for point-of-care biosensors. Thus, electrical bio-assays on paper-based microfluidics can be realized to develop low cost, sensitive, and specific diagnostic devices.
- Subjects :
- Animals
Avidin analysis
Biotin analysis
Cattle
Dimethylpolysiloxanes chemistry
Electricity
Hydrophobic and Hydrophilic Interactions
Immobilized Proteins analysis
Reference Standards
Reproducibility of Results
Serum Albumin, Bovine analysis
Solutions
Biosensing Techniques instrumentation
Microfluidic Analytical Techniques instrumentation
Nanotubes, Carbon chemistry
Paper
Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 2694-0604
- Volume :
- 2013
- Database :
- MEDLINE
- Journal :
- Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
- Publication Type :
- Academic Journal
- Accession number :
- 24109651
- Full Text :
- https://doi.org/10.1109/EMBC.2013.6609464