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In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2013 Dec 01; Vol. 33 (8), pp. 5008-18. Date of Electronic Publication: 2013 Sep 07. - Publication Year :
- 2013
-
Abstract
- Hydroxyapatite (HAp), with its high biocompatibility and osteoconductivity, readily absorbs proteins, amino acids and other substances, which in turn favor the adsorption and colonization of bacteria. To prevent bacterial growth and biofilm formation on HAp discs, silver-containing (1-20 mol%) HAp (Ag-HAp) powders were synthesized using an ultrasonic spray pyrolysis (USSP) technique. The X-ray diffraction (XRD) peaks were very broad, indicating low crystallinity, and this induced the release of Ag(+) ions from Ag-HAp powders. In addition, a gradual increase in Ca(2+) ion release was observed. These results suggest that dissolution of Ca(2+) ion in Ag-HAp triggered the release of Ag(+) ions. The antimicrobial efficacy of Ag-HAp disc was tested against Staphylococcus aureus. Samples with Ag contents of more than 5 mol% were found to be highly effective against bacterial colonization and biofilm formation in vitro. In vivo antibacterial tests using bioluminescent strains also showed reductions in the viability of bacteria with Ag-HAp (5 mol%) discs. Biocompatibility tests using a modified Transwell® insert method showed that Ag-HAp (5 mol%) discs have negative effects on osteoblast proliferation. These results indicate that Ag-HAp (5 mol%) has effective antibacterial activity and good biocompatibility both in vitro and in vivo together with good biocompatibility, thus confirming its utility as a bactericidal material.<br /> (© 2013.)
- Subjects :
- Animals
Anti-Infective Agents pharmacology
Biofilms drug effects
Calcium chemistry
Cell Line
Cell Proliferation drug effects
Coated Materials, Biocompatible pharmacology
Ions chemistry
Luminescent Measurements
Male
Materials Testing
Mice
Mice, Inbred BALB C
Osteoblasts cytology
Prostheses and Implants
Silver pharmacology
Sonication
Staphylococcal Infections pathology
Staphylococcus aureus drug effects
Staphylococcus aureus physiology
Anti-Infective Agents chemistry
Coated Materials, Biocompatible chemistry
Durapatite chemistry
Silver chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 33
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 24094218
- Full Text :
- https://doi.org/10.1016/j.msec.2013.08.026