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Evidence that Notch and Delta expressions have a role in dermal condensate aggregation during wool follicle initiation.
- Source :
-
Experimental dermatology [Exp Dermatol] 2013 Oct; Vol. 22 (10), pp. 659-62. - Publication Year :
- 2013
-
Abstract
- Notch pathway genes have been implicated in the commitment of mesenchymal cells to a wool follicle cell fate. Notch1 and Delta1 transcripts were quantified in fetal skin of fine-woolled (Merino) and strong-woolled (Tukidale) sheep at two time points: either preceding (d56) or during (d70) the first wave of follicle initiation. DIG-labelled probes for both transcripts were localised in the epithelium, some mesenchymal cells, and in the dermal condensates of primordia. The possibility that condensates selectively incorporated Delta1-labelled mesenchymal cells is considered. The involvement of Notch1 in condensate formation was also explored in cultured fetal skin explants and whisker papilla cells using DAPT to block Notch signalling. In its presence, follicle initiation in skin explants was reduced, and the propensity for cultured papilla cells to aggregate was abolished. Results suggest that Notch1 activation is a prerequisite for mesenchymal aggregation. It is speculated that Delta interactions contribute to condensate formation, in vivo.<br /> (© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Cell Differentiation
Mesoderm cytology
Receptor, Notch1 metabolism
Sheep
Signal Transduction
Skin pathology
Skin Physiological Phenomena
Time Factors
Wool
Gene Expression Regulation, Developmental
Hair Follicle metabolism
Intracellular Signaling Peptides and Proteins metabolism
Membrane Proteins metabolism
Receptors, Notch metabolism
Skin embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0625
- Volume :
- 22
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Report
- Accession number :
- 24079736
- Full Text :
- https://doi.org/10.1111/exd.12217