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Effect of 48-h food deprivation on the expressions of myosin heavy-chain isoforms and fiber type-related factors in rats.
- Source :
-
Journal of nutritional science and vitaminology [J Nutr Sci Vitaminol (Tokyo)] 2013; Vol. 59 (4), pp. 289-98. - Publication Year :
- 2013
-
Abstract
- The primary aim of this study was to examine the effects of 48-h food deprivation on rat skeletal muscle fiber type, according to myosin heavy-chain (MyHC) isoform composition and some metabolism-related factors in both slow-type dominant and fast-type dominant muscle tissues. Male Wistar rats (7 wk old) were treated with 48-h food deprivation or ad libitum feeding as control. After the treatment, the soleus muscle (slow-type dominant) and the extensor digitorum longus (EDL, fast-type dominant) were excised. We found that 48-h food deprivation did not affect MyHC composition in either the soleus or EDL, compared with fed rats by electrophoretic separation of MyHC isoforms. However, 48-h food deprivation significantly increased the mRNA expression of fast-type MyHC2B in the EDL muscle. Moreover, food deprivation increased fatty acid metabolism, as shown by elevated levels of related serum energy substrates and mRNA expression of mitochondrial uncoupling protein (UCP) 3 and lipoprotein lipase (LPL) in both the soleus and EDL. UCP3 and LPL are generally expressed at higher levels in slow-type fibers. Furthermore, we found that food deprivation significantly decreased the protein amounts of PGC1α and phosphorylated FOXO1, which are known as skeletal muscle fiber type regulators. In conclusion, 48-h food deprivation increased mRNA expression of fast-type MyHC isoform and oxidative metabolism-related factors in EDL, whereas MyHC composition at the protein level did not change in either the soleus or EDL.
- Subjects :
- Animals
Forkhead Transcription Factors metabolism
Ion Channels genetics
Ion Channels metabolism
Lipoprotein Lipase genetics
Lipoprotein Lipase metabolism
Male
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Muscle, Skeletal metabolism
Nerve Tissue Proteins metabolism
Oxidation-Reduction
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Phosphorylation
Protein Isoforms
RNA, Messenger metabolism
Rats
Rats, Wistar
Transcription Factors genetics
Uncoupling Protein 3
Fatty Acids metabolism
Food Deprivation physiology
Muscle Fibers, Fast-Twitch metabolism
Muscle Fibers, Slow-Twitch metabolism
Myosin Heavy Chains metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1881-7742
- Volume :
- 59
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of nutritional science and vitaminology
- Publication Type :
- Academic Journal
- Accession number :
- 24064729
- Full Text :
- https://doi.org/10.3177/jnsv.59.289