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Elevated 3-hydroxypropionaldehyde production from glycerol using a Citrobacter freundii mutant.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2014 Jan; Vol. 36 (1), pp. 147-52. Date of Electronic Publication: 2013 Sep 24. - Publication Year :
- 2014
-
Abstract
- A mutant strain of Citrobacter freundii capable of elevated 3-hydroxypropionaldehyde production from glycerol was isolated using chemical mutagenesis and a screening protocol. The protocol involved screening mutagenized bacterial cells on solid minimal medium containing 5 % (v/v) glycerol. Colonies were picked onto duplicate solid minimal medium plates and one plate was stained with 1 % (w/v) phloroglucinol. Those colonies staining red were further screened and a mutant, HPAO-1, was identified. The mutant strain produced a several-fold higher 3-hydroxypropionaldehyde concentration than did the parent strain when grown on 5 % (v/v) glycerol. The ratio of culture volume to flask volume influenced 3-hydroxypropionaldehyde production by the mutant cells compared to the parent cells. Aldehyde production was highest when the mutant strain was grown on 5 % (v/v) glycerol at a ratio of culture volume to flask volume of 1:3 or 1:12.5.
- Subjects :
- Biomass
Citrobacter freundii metabolism
Coloring Agents chemistry
Coloring Agents metabolism
Glyceraldehyde analysis
Glyceraldehyde metabolism
Mutation
Phloroglucinol chemistry
Phloroglucinol metabolism
Citrobacter freundii genetics
Glyceraldehyde analogs & derivatives
Glycerol metabolism
Propane analysis
Propane metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 24062134
- Full Text :
- https://doi.org/10.1007/s10529-013-1343-y